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University of Tartu

2 ARTICLES PUBLISHED IN JoVE

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Engineering

Fabrication of Carbon-Based Ionic Electromechanically Active Soft Actuators
Pille Rinne 1, Inga Põldsalu 1, Herman Klas Ratas 1, Karl Kruusamäe 1, Urmas Johanson 1, Tarmo Tamm 1, Kaija Põhako-Esko 1, Andres Punning 1, Anna-Liisa Peikolainen 1, Friedrich Kaasik 1, Indrek Must 1, Daan van den Ende 2, Alvo Aabloo 1
1Intelligent Materials and Systems Lab, Institute of Technology, University of Tartu, 2Smart Interfaces & Modules Department, Philips Research

This article describes a fast and simple manufacturing process of ionic electromechanically active composite materials for actuators in biomedical, biomimetic and soft robotics applications. The key fabrication steps, their importance for the actuators' final properties, and some of the main characterization techniques are described in detail.

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Neuroscience

Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity
Alistair Gamble 1, Maria Suessmilch 1, Anniek Bonestroo 1, Andres Merits 2, Gerard J. Graham 1, Jonathan Cavanagh 1, Julia M. Edgar 1, Marieke Pingen 1
1School of Infection and Immunity, University of Glasgow, 2Faculty of Science and Technology, Institute of Technology, University of Tartu

This protocol presents a unique way of generating central nervous system cell cultures from embryonic day 17 mouse brains for neuro(immuno)logy research. This model can be analyzed using various experimental techniques, including RT-qPCR, microscopy, ELISA, and flow cytometry.

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