Gamete Collection and In Vitro Fertilization of Astyanax mexicanusRobert Peuß *1, Zachary Zakibe *1, Jaya Krishnan 1, M. Shane Merryman 1, Diana P. Baumann 1, Nicolas Rohner 1,2
1Stowers Institute for Medical Research, 2Department of Molecular & Integrative Physiology, KU Medical Center
In vitro fertilization is a commonly used technique with a variety of model organisms to maintain lab populations and produce synchronized embryos for downstream applications. Here, we present a protocol that implements this technique for different populations of the Mexican tetra fish, Astyanax mexicanus.
