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NYU Langone Medical Center

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
Rachel Lubong Sabado 1, Elizabeth Miller 2, Meredith Spadaccia 1, Isabelita Vengco 1, Farah Hasan 1, Nina Bhardwaj 1
1Cancer Institute, NYU Langone Medical Center, 2Infectious Diseases, NYU Langone Medical Center

The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.

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Immunology and Infection

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Melissa V. Fernandez 1, Elizabeth A. Miller 2, Nina Bhardwaj 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.

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Medicine

A Protocol for the Use of Remotely-Supervised Transcranial Direct Current Stimulation (tDCS) in Multiple Sclerosis (MS)
Margaret Kasschau 1,2, Kathleen Sherman 1,2, Lamia Haider 2, Ariana Frontario 1,2, Michael Shaw 1,2, Abhishek Datta 3, Marom Bikson 4, Leigh Charvet 1,2
1Multiple Sclerosis Comprehensive Care Center, Department of Neurology, NYU Langone Medical Center, 2Department of Neurology, Stony Brook Medicine, 3Soterix Medical, Inc, 4Department of Biomedical Engineering, The City College of New York

The goal of this pilot study is to describe a protocol for the remotely-supervised delivery of transcranial direct current stimulation (tDCS) so that the procedure maintains standards of in-clinic practice, including safety, reproducibility, and tolerability. The feasibility of this protocol was tested in participants with multiple sclerosis (MS).

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Neuroscience

Remotely Supervised Transcranial Direct Current Stimulation: An Update on Safety and Tolerability
Michael T. Shaw 1, Margaret Kasschau 2, Bryan Dobbs 1, Natalie Pawlak 1, William Pau 1, Kathleen Sherman 1, Marom Bikson 3, Abhishek Datta 4, Leigh E. Charvet 1
1New York University, Langone Medical Center, 2Stony Brook Medicine, 3City College of New York, 4Soterix Medical

This manuscript provides an updated remote supervision protocol that enables participation in transcranial direct current stimulation (tDCS) clinical trials while receiving treatment sessions from home. The protocol has been successfully piloted in both patients with multiple sclerosis and Parkinson's disease.

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