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University of Sassari

3 ARTICLES PUBLISHED IN JoVE

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Behavior

Behavioral Disturbances: An Innovative Approach to Monitor the Modulatory Effects of a Nutraceutical Diet
Alessandro Di Cerbo *1, Sara Sechi *2, Sergio Canello 3, Gianandrea Guidetti 3, Filippo Fiore 2, Raffaella Cocco 2
1School of Specialization in Clinical Biochemistry, “G. d’Annunzio” University, 2Department of Veterinary Medicine, Pathology and Veterinary Clinic Section, University of Sassari, 3Research and Development Department, Forza10 USA Corp.

We report a simple approach to evaluate the effectiveness of a specific diet in positively modulating the daily activity and clinical and behavioral symptoms of dogs with evident behavioral disturbances.

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Biology

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
Georgia Pennarossa 1, Sergio Ledda 2, Sharon Arcuri 1, Fulvio Gandolfi 3, Tiziana A. L. Brevini 1
1Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università degli Studi di Milano, 2Department of Veterinary Medicine, University of Sassari, 3Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy and Center for Stem Cell Research, Università degli Studi di Milano

We here present a method that combines the use of chemical epigenetic erasing with mechanosensing-related cues to efficiently generate mammalian pluripotent cells, without the need of gene transfection or retroviral vectors. This strategy is, therefore, promising for translational medicine and represents a notable advancement in stem cell organoid technology.

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Developmental Biology

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Sara Succu 1, Elisa Serra 1, Sergio Gadau 1, Antonio Varcasia 1, Fiammetta Berlinguer 1
1Department of Veterinary Medicine, University of Sassari

The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.

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