Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk MetabolomicsDarian T. Carroll 1, Sarah R. Lindsley 2, Melissa Kirigiti 2, Alex Buko 3, Angela Jones 4, Paul Kievit 2, Maureen Gannon 1,5,6,7
1Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Metabolic Health and Disease, Oregon National Primate Research Center, 3Human Metabolome Technologies (HMT) America, 4Vanderbilt Technologies for Advanced Genomics, Vanderbilt University Medical Center, 5Department of Medicine, Vanderbilt University Medical Center, 6Department of Veterans Affairs Tennessee Valley, 7Department of Cell and Developmental Biology, Vanderbilt University
This protocol describes procedures to isolate high-quality nuclei from frozen non-human primate pancreatic islets for use in single-nucleus simultaneous RNA sequencing and ATAC sequencing while preserving the bulk cytosolic fraction for metabolomic analyses from the same samples.
