EMD Chemicals Inc.

2 ARTICLES PUBLISHED IN JoVE

Biology

Western Blotting: Sample Preparation to Detection

¹Research and Development, EMD Chemicals Inc.

Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.

Biology

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Carol Buchner¹, Cassandra Bryant², Anna Eslami³, Gabriella Lakos⁴

¹IFA Development Manager, INOVA Diagnostics, Inc., ²IFA Development, INOVA Diagnostics, Inc., ³Product Manager, Rheumatology, INOVA Diagnostics, Inc., ⁴Medical Director, INOVA Diagnostics, Inc.

Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.