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Universidad Nacional de Colombia

4 ARTICLES PUBLISHED IN JoVE

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Behavior

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats
César Acevedo-Triana 1,6, Javier L. Rico 2, Leonardo A. Ortega 2, Melissa Andrea N. Cardenas 3, Fernando P. Cardenas 3, Manuel J. Rojas 4, Juan Carlos Forigua-Vargas 2, Julián Cifuentes 2, Camilo Hurtado-Parrado 2,5
1School of Psychology, Universidad Pedagógica y Tecnológica de Colombia, 2Animal Behavior Laboratory, Fundación Universitaria Konrad Lorenz, 3Department of Psychology, Universidad de Los Andes, 4School of Veterinary Medicine, Animal Health Department, Universidad Nacional de Colombia, 5Department of Psychology, Troy University, 6Department of Neurobiology, University of Alabama at Birmingham

We describe an extended fear-conditioning protocol that produces overtraining and fear incubation in rats. This protocol entails a single training session with 25 tone-shock pairings (i.e., overtraining) and a comparison of conditioned freezing responses during context and cue tests 48 h (short-term) and 6 weeks (long-term) after training.

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Neuroscience

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells
Carolina Galeano-Naranjo 1,2, A. Catalina Veléz-Ortega 1, Gregory I Frolenkov 1
1Department of Physiology, College of Medicine, University of Kentucky, 2Universidad Nacional de Colombia

Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells.

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JoVE Core

Electric and Magnetic Field Devices for Stimulation of Biological Tissues
Juan José Saiz Culma 1,2, Juan Felipe Escobar Huertas 2,3, Diego Alexander Garzón-Alvarado 1,2, Juan Jairo Vaca-Gonzalez 1,4
1Biomimetics Laboratory, Instituto de Biotecnología, Universidad Nacional de Colombia, 2Numerical Methods and Modeling Research Group (GNUM), Universidad Nacional de Colombia, 3Design, Analysis and Development of Engineering Systems Research group (GIDAD), Fundación Universitaria Los Libertadores, 4School of Health and Sports Sciences, Master Program in Epidemiology, Fundación Universitaria del Área Andina

This protocol describes the step-by-step process to build both electrical and magnetic stimulators used to stimulate biological tissues. The protocol includes a guideline to simulate computationally electric and magnetic fields and manufacture of stimulator devices.

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Developmental Biology

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
Daniela Londoño-Vásquez 1,2, Alexander Jurkevich 3, Ahmed Z. Balboula 1
1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri

An optimized protocol is presented that enables the depletion of cytoplasmic microtubule organizing centers in mouse oocytes during metaphase I using a near-infrared femtosecond laser.

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