Techniques for the Analysis of Extracellular Vesicles Using Flow CytometryHeather Inglis 1, Philip Norris 1,2,3, Ali Danesh 1,3
1Blood Systems Research Institute, 2Department of Medicine, University of California, San Francisco, 3Department of Laboratory Medicine, University of California, San Francisco
Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.