Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell CulturePedro Gameiro dos Santos 1, Ana Rita Soares 1, Sólveig Thorsteinsdóttir 1, Gabriela Rodrigues 1
1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences, University of Lisbon
In this work, a decellularization protocol was optimized to obtain decellularized matrices of fetal mouse skeletal muscle. C2C12 myoblasts can colonize these matrices, proliferate, and differentiate. This in vitro model can be used to study cell behavior in the context of skeletal muscle diseases such as muscular dystrophies.
