JoVE Logo

Sign In

Health Canada

4 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Caroline Gravel *1, Changgui Li *2, Junzhi Wang 2, Anwar M Hashem 1,3,4, Bozena Jaentschke 1, Gary Van Domselaar 5, Runtao He 5, Xuguang Li 1,3
1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada

A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.

image

Engineering

Fabrication of VB2/Air Cells for Electrochemical Testing
Jessica Stuart 1, Ruben Lopez 2, Jason Lau 1, Xuguang Li 2, Mahesh Waje 2, Matthew Mullings 2, Christopher Rhodes 2, Stuart Licht 1
1Department of Chemistry, The George Washington University, 2Lynntech

A protocol is presented to study multi-electron metal/air battery systems by using previous technology developed for the zinc/air cell. Electrochemical testing is then performed on fabricated batteries to evaluate performance.

image

Biology

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
Jason M. O'Brien 1, Marc A. Beal 1, John D. Gingerich 1, Lynda Soper 1, George R. Douglas 1, Carole L. Yauk 1, Francesco Marchetti 1
1Environmental Health Science and Research Bureau, Health Canada, Environmental Health Centre

De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.

image

Chemistry

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
Nikolai L. Chepelev 1, Dean A. Kennedy 1, Remi Gagné 1, Taryn White 1, Alexandra S. Long 1, Carole L. Yauk 1, Paul A. White 1
1Environmental Health Science and Research Bureau, Health Canada

The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved