Health Canada

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

Caroline Gravel^*1, Changgui Li^*2, Junzhi Wang², Anwar M Hashem^1,3,4, Bozena Jaentschke¹, Gary Van Domselaar⁵, Runtao He⁵, Xuguang Li^1,3

¹Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, ²National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, ³Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ⁴Microbiology Department, Faculty of Medicine, King Abdulaziz University, ⁵National Microbiology Laboratory, Public Health Agency of Canada

A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.

Engineering

Fabrication of VB₂/Air Cells for Electrochemical Testing

Jessica Stuart¹, Ruben Lopez², Jason Lau¹, Xuguang Li², Mahesh Waje², Matthew Mullings², Christopher Rhodes², Stuart Licht¹

¹Department of Chemistry, The George Washington University, ²Lynntech

A protocol is presented to study multi-electron metal/air battery systems by using previous technology developed for the zinc/air cell. Electrochemical testing is then performed on fabricated batteries to evaluate performance.

Biology

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Jason M. O'Brien¹, Marc A. Beal¹, John D. Gingerich¹, Lynda Soper¹, George R. Douglas¹, Carole L. Yauk¹, Francesco Marchetti¹

¹Environmental Health Science and Research Bureau, Health Canada, Environmental Health Centre

De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.

Chemistry

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Nikolai L. Chepelev¹, Dean A. Kennedy¹, Remi Gagné¹, Taryn White¹, Alexandra S. Long¹, Carole L. Yauk¹, Paul A. White¹

¹Environmental Health Science and Research Bureau, Health Canada

The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.