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Friedrich-Loeffler-Institut

4 ARTICLES PUBLISHED IN JoVE

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Medicine

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques
Annette Prohl 1, Carola Ostermann 1, Markus Lohr 1, Petra Reinhold 1
1Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut

This article describes bronchoscopic techniques in the bovine lung under experimental conditions, i.e. bronchoscopically guided inoculation, bronchoalveolar lavage, bronchial brushing, and transbronchial lung biopsy.

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Immunology and Infection

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
Thomas Hoenen 1, Ari Watt 1, Anita Mora 2, Heinz Feldmann 1
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

Work with infectious Ebola viruses is restricted to biosafety level 4 laboratories. Tetracistronic minigenome-containing replication and transcription-competent virus like particles (trVLPs) represent a lifecycle modeling system that allows us to safely model multiple infectious cycles under biosafety level 2 conditions, relying exclusively on Ebola virus components.

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Immunology and Infection

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Christian Berens 1,2, Stephanie Bisle 3, Leonie Klingenbeck 3, Anja Lührmann 3
1Department Biologie, Friedrich-Alexander-Universität, 2Institut für Molekulare Pathogenese, Friedrich-Loeffler-Institut, 3Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen

The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.

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Genetics

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Andreas Mueller 1, Kerstin Fischer 2, Roland Suluku 3, Thomas Hoenen 1
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 2Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, 3Department of Animal Science, Njala University

Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.

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