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Tel-Aviv University

13 ARTICLES PUBLISHED IN JoVE

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Medicine

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells
Asher Castiel 1, Leonid Visochek 2,3, Leonid Mittelman 4, Yael Zilberstein 4, Francoise Dantzer 5, Shai Izraeli 1,6, Malka Cohen-Armon 2,3
1Cancer Research Center, Sheba Medical Center, 2The Neufeld Cardiac Research Institute, Tel-Aviv University, 3Department of Physiology and Pharmacology, Tel-Aviv University, 4Imaging Unit, Sackler Faculty of Medicine, Tel-Aviv University, 5Biotechnology and Cell Signaling, Ecole Superieure de Biotechnologie Strasbourg, 6Department of Human Molecular Genetics and Biochemistry, Tel-Aviv University

The cytotoxic activity of the phenanthridine PJ-34 in cancer cells undergoing mitosis was documented in real time by live confocal imaging. PJ-34 eradicated human breast cancer MDA-MB-231 cells harboring extra-centrosomes in mitosis. Unlike normal bi-focal mitosis, the extra-centrosomes were not clustered in the two spindle poles in the presence of PJ-34.

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Neuroscience

Extracting Visual Evoked Potentials from EEG Data Recorded During fMRI-guided Transcranial Magnetic Stimulation
Boaz Sadeh 1, Galit Yovel 1,2
1School of Psychological Sciences, Tel-Aviv University, 2Sagol School of Neuroscience, Tel-Aviv University

This paper describes a method for collecting and analyzing electroencephalography (EEG) data during concurrent transcranial magnetic stimulation (TMS) guided by activations revealed with functional magnetic resonance imaging (fMRI). A method for TMS artifact removal and extraction of event related potentials is described as well as considerations in paradigm design and experimental setup.

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Behavior

Signal Attenuation as a Rat Model of Obsessive Compulsive Disorder
Koral Goltseker *1, Roni Yankelevitch-Yahav *1, Noa S. Albelda *1, Daphna Joel 1,2
1School of Psychological Sciences, Tel-Aviv University, 2Sagol School of Neuroscience, Tel-Aviv University

The goal of the protocol described in this paper is to induce compulsive-like behavior in rats for the study of obsessive-compulsive disorder (OCD). This behavior is precipitated by attenuating a signal indicating that a lever-press response was effective in producing food.

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Neuroscience

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
Sivan Kanner *1, Marta Bisio *2, Gilad Cohen 3, Miri Goldin 4, Marieteresa Tedesco 5, Yael Hanein 3, Eshel Ben-Jacob 4, Ari Barzilai 1, Michela Chiappalone 2, Paolo Bonifazi 1,4
1Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, 2Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 3School of Electrical Engineering, Tel-Aviv University, 4School of Physics and Astronomy, Tel-Aviv University, 5Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova

This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.

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Behavior

The Forced Swim Test as a Model of Depressive-like Behavior
Roni Yankelevitch-Yahav 1,2, Motty Franko 2, Avrham Huly 2, Ravid Doron 2,3,4
1School of Psychological Sciences, Tel-Aviv University, 2School of Behavioral Sciences, Academic College of Tel Aviv-Yaffo, 3Department of Education and Psychology, The Open University of Israel, 4School of Health and Life Sciences, Hadassah Academic College

This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral despair.

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Behavior

The Emotional Stroop Task: Assessing Cognitive Performance under Exposure to Emotional Content
Moshe Shay Ben-Haim 1, Paul Williams 2, Zachary Howard 2, Yaniv Mama 3, Ami Eidels 2, Daniel Algom 1
1School of Psychological Sciences, Tel-Aviv University, 2School of Psychology, University of Newcastle, 3Department of Behavioral Sciences, Ariel University

The emotional Stroop effect (ESE) is the result of longer naming latencies to ink colors of emotion words than those of neutral words. This report refers to potential sources of confounding and includes a modal experiment that provides the means to control for them.

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Immunology and Infection

Selective Harvesting of Marginating-pulmonary Leukocytes
Lee Shaashua *1, Liat Sorski *1, Rivka Melamed 1, Shamgar Ben-Eliyahu 1
1Sagol School of Neuroscience & School of Psychological Sciences, Tel-Aviv University

Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases.

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Immunology and Infection

Selective Harvesting of Marginating-hepatic Leukocytes
Liat Sorski *1, Lee Shaashua *1, Rivka Melamed 1, Pini Matzner 1, Shamgar Ben-Eliyahu 1
1Sagol School of Neuroscience & School of Psychological Sciences, Tel-Aviv University

Marginating-hepatic leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe a method for selective harvesting of this specific hepatic cell population, through forced perfusion of the liver of rats or mice. Marginating-hepatic leukocytes seem critical in determining susceptibility to hepatic-related diseases and metastases.

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Behavior

Using Virtual Reality to Transfer Motor Skill Knowledge from One Hand to Another
Ori Ossmy 1,2, Roy Mukamel 1,2
1Sagol School of Neuroscience, Tel-Aviv University, 2School of Psychological Sciences, Tel-Aviv University

We describe a novel virtual-reality based setup which exploits voluntary control of one hand to improve motor-skill performance in the other (non-trained) hand. This is achieved by providing real-time movement-based sensory feedback as if the non-trained hand is moving. This new approach may be used to enhance rehabilitation of patients with unilateral hemiparesis.

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Education

Measurements of Waves in a Wind-wave Tank Under Steady and Time-varying Wind Forcing
Andrey Zavadsky 1, Lev Shemer 1
1School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University

This manuscript describes a fully computer-controlled procedure that allows obtaining reliable statistical parameters from experiments of water waves excited by steady and unsteady wind forcing in a small-scale facility.

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Cancer Research

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Nadine Santana-Magal *1, Diana Rasoulouniriana *1, Corey Saperia 1, Amit Gutwillig 1, Peleg Rider 1, Edgar G. Engleman 2, Yaron Carmi 1
1Department of Pathology, Sackler School of Medicine, Tel-Aviv University, 2Department of Pathology, School of Medicine, Stanford University

Monocyte-derived DC (MoDC) can sense minor amounts of danger-associated molecules and are therefore easily primed. We provide a detailed protocol for the isolation of MoDC from blood and tumors and their activation with immune complexes while highlighting key precautions that should be considered in order to avoid their premature activation.

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Engineering

Measurements of Local Instantaneous Convective Heat Transfer in a Pipe - Single and Two-phase Flow
Adam Fershtman 1, Dvora Barnea 1, Lev Shemer 1
1School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University

This manuscript describes methods aimed at measuring the local instantaneous convective heat transfer coefficients in a single or two-phase pipe flow. A simple optical method to determine the length and the propagation velocity of an elongated (Taylor) air bubble moving at a constant velocity is also presented.

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Bioengineering

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
Avraham Kolel 1, Avishy Roitblat Riba 2, Sari Natan 3, Oren Tchaicheeyan 3, Eilom Saias 2, Ayelet Lesman 3,4
1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University

The presented method involves uniaxial stretching of 3D soft hydrogels embedded in silicone rubber while allowing live confocal microscopy. Characterization of the external and internal hydrogel strains as well as fiber alignment are demonstrated. The device and protocol developed can assess the response of cells to various strain regimes.

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