A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection ModelOfelia Muñoz-Paleta *1, Enrique Olguín-Martínez *1, Blanca Estela Ruiz-Medina *1, Alejandra Alonso-Quintana 1, Ma Cleofas Marcial-Medina 1, Paula Licona-Limón 1
1Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México
IL-9-expressing T and ILC2 cells are induced during N. brasiliensis infection, yet their characterization has been largely overlooked in the infected intestine due to their low frequency and differential kinetics. This protocol describes the isolation of these cells from different target organs and confirmation of their identity via flow cytometry at different infection stages.
