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University of Texas at Tyler

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
Anna M. Biesbrock 1, Christopher M. Powell 1, Wayne B. Hunter 2, Blake R. Bextine 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.

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