University of Texas at Tyler

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

Anna M. Biesbrock¹, Christopher M. Powell¹, Wayne B. Hunter², Blake R. Bextine¹

¹Department of Biology, University of Texas at Tyler, ²U. S. Horticultural Research Laboratory, USDA ARS

Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.