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BC Centre for Excellence in HIV/AIDS

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Rachel A. McGovern 1, P. Richard Harrigan 1, Luke C. Swenson 1
1Laboratory Program, BC Centre for Excellence in HIV/AIDS

HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5 virus.

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Inferencia Genotipica de tropismo VIH-1 usando secuanciacion de V3 basada en poblacion - ADVERTISEMENT
Rachel A. McGovern 1, P. Richard Harrigan 1, Luke C. Swenson 1
1Laboratory Program, BC Centre for Excellence in HIV/AIDS

Inferencia Genotipica de tropismo VIH-1 usando secuanciacion de V3 basada en poblacion - ADVERTISEMENT

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