HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5 virus.
Inferencia Genotipica de tropismo VIH-1 usando secuanciacion de V3 basada en poblacion - ADVERTISEMENT
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved