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Universidade Estadual Paulista (UNESP)

1 ARTICLES PUBLISHED IN JoVE

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Biology

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Camila B. Giuliano 1,2, Rongjing Zhang 1, Laurence G. Wilson 1
1The Rowland Institute, Harvard University, 2Faculdade de Ciências e Letras de Assis, Universidade Estadual Paulista

The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.

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