The MITRE Corporation

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

M. Heath Farris¹, Kara A. Ford², Richard C. Doyle³

¹Department of Advanced Technology, The MITRE Corporation, ²Center for Biomedical Engineering, Brown University, ³The MITRE Corporation

Here, we present protocols to rapidly screen and characterize enzymes for antimicrobial activity. The microslide diffusion assay and the dye-release assay utilize target bacterial substrates for qualitative and quantitative enzymatic activity evaluation.