Fisk University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Expression, Purification, and Antimicrobial Activity of S100A12

Emmanuel Jackson¹, Saffron Little¹, Dana S. Franklin¹, Jennifer A. Gaddy^2,3, Steven M. Damo^1,4

¹Department of Life and Physical Sciences, Fisk University, ²Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs, ³Department of Medicine - Division of Infectious Diseases, Vanderbilt University Medical School, ⁴Department of Biochemistry, Vanderbilt University

Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.