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Lake Forest College

2 ARTICLES PUBLISHED IN JoVE

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Biology

Experimental Manipulation of Body Size to Estimate Morphological Scaling Relationships in Drosophila
R. Craig Stillwell 1, Ian Dworkin 2, Alexander W. Shingleton 2, W. Anthony Frankino 1
1Department of Biology & Biochemistry, University of Houston, 2Department of Zoology, Michigan State University

Morphological scaling relationships capture and describe organismal shape. We present a method to measure morphological scaling relationships across the natural range of body sizes in fully metamorphic insects. Using a simple diet manipulation we increase the distribution of trait sizes, permitting the accurate description of how shape and size co-vary.

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Genetics

Quantifying Abdominal Pigmentation in Drosophila melanogaster
Omid Saleh Ziabari 1, Alexander W. Shingleton 1
1Department of Biology, Lake Forest College

This work presents a method to quickly and precisely quantify the abdominal pigmentation of Drosophila melanogaster using digital image analysis. This method streamlines the procedures between phenotype acquisition and data analysis and includes specimen mounting, image acquisition, pixel value extraction, and trait measurement.

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