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Ouachita Baptist University

2 ARTICLES PUBLISHED IN JoVE

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Biology

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
Jace C. Bradshaw 1, Allea Belle Gongola 1, Nathan S. Reyna 1
1Department of Biology, Ouachita Baptist University

This protocol utilizes Golden Gate Assembly and the plasmid pGR-blue to rapidly quantify the strength of terminators found in silico.

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Biology

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection
Elijah I. Christenson 1, Qingyang Zhang 2, Ruth Plymale 1
1Department of Biology, Ouachita Baptist University, 2Department of Mathematical Sciences, University of Arkansas

An optical density-based microplate method is described to quantify long-term bacterial growth in the presence of bacteriophages, suitable for actinomycetes and other slow-growing bacteria. This method includes modifications to reduce evaporation and lid condensation and R code to calculate virus infection metrics, including the area under the curve, growth maximum, and relative virulence.

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