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Western Washington University

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

A General Method for Evaluating Incubation of Sucrose Craving in Rats
Jeffrey W. Grimm 1, Jesse Barnes 1, Kindsey North 1, Stefan Collins 1, Rachel Weber 1
1Department of Psychology and Program in Behavioral Neuroscience, Western Washington University

Responding for food or drug-paired cues increases over the course of a period of abstinence and this may relate to an increased susceptibility to relapse behaviors. Here we detail a procedure for evaluating this "incubation of craving" in rats that have self-administered sucrose.

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Biology

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
Graham Bailes 1, Margaret Lind 1, Andrew Ely 1, Marianne Powell 2, Jennifer Moore-Kucera 3, Carol Miles 2, Debra Inglis 2, Marion Brodhagen 1
1Biology Department, Western Washington University, 2Washington State University Northwestern Research and Extension Center, 3Department of Plant and Soil Science, Texas Tech University

Plastic films labeled "biodegradable" are commercially available for agricultural use as mulches. Tillage represents an attractive disposal method, but degradation under field conditions is poorly understood. The purpose of this study was to develop methods for isolating native soil fungi and bacteria that colonize plastic mulch films after field burial.

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Environment

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
Gregory W. O'Neil 1, John R. Williams 1, Julia Wilson-Peltier 1, Gerhard Knothe 2, Christopher M. Reddy 3
1Department of Chemistry, Western Washington University, 2Agricultural Research Service, United States Department of Agriculture, 3Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution

Detailed methods are presented for the production of biodiesel along with the co-isolation of alkenones as valuable coproducts from commercial Isochrysis microalgae.

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Chemistry

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
Xinyi Ding 1,2,3, Yuxiang Sun 1,2,3, Yanming Chen 1,2,3, Wanchuan Ding 1,2,3, Steven Emory 1,2,3,4, Tianhao Li 1,2,3, Zixing Xu 1,2,3, Ning Han 1,2,3, Jun Wang 1,2,3, Gang Ruan 1,2,3
1Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, 2Institute of Materials Engineering, College of Engineering and Applied Sciences, Nanjing University, 3Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, 4Department of Chemistry, Western Washington University

The present work describes a method to fabricate micellar nanocrystals, an emerging major class of nanobiomaterials. This method combines top-down electrospray, bottom-up self-assembly, and solvent-based structure control. The fabrication method is largely continuous, can produce high quality products, and possesses an inexpensive means of structure control.

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