Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture InsertsLinnea Gustafsson *1,2, Savvini Gkouma *3, Alexander Jönsson 4, Martin Dufva 4, My Hedhammar 3
1Spiber Technologies AB, 2Division of Micro and Nanosystems, KTH Royal Institute of Technology, 3Division of Protein Technology, KTH Royal Institute of Technology, 4Department of Health Technology, Technical University of Denmark
Tissue culture inserts with plastic membranes are the golden standard in cell culture labs as permeable supports to establish cell layers and models of barrier tissues. Herein, we present a simple method to replace the plastic membrane with a more biologically relevant membrane made from a recombinant functionalized spider silk protein.
