Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver SlicesDany Perocheau 1, Claire Duff 1, Sonam Gurung 1, Loukia Touramanidou 1, Garima Sharma 1, Julien Baruteau 1,2,3
1Great Ormond Street Institute of Child Health, University College London, 2Great Ormond Street Hospital for Children NHS Foundation Trust, 3National Institute of Health Research Great Ormond Street Biomedical Research Centre
A protocol for the production and culture of Precision-cut Liver Slices (PCLS) for the study of mouse livers. The article focuses on key aspects of the protocol, which only requires standard laboratory equipment with access to a vibratome and allows survival of PCLS for a minimum of 4 days.
