University of Zagreb School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Optimization of the Longa Middle Cerebral Artery Occlusion Method for Complete Reperfusion

Rok Ister¹, Marta Pongrac¹, Marina Dobrivojević Radmilović^1,2

¹Department of Histology and Embryology, University of Zagreb School of Medicine, ²Croatian institute for brain research, University of Zagreb School of Medicine

This protocol presents a step-by-step guide for researchers to perform the middle cerebral artery occlusion procedure on mice using the modified Longa external carotid artery method. Modifications presented in this article aim to increase the accuracy of middle cerebral artery occlusion and ensure complete reperfusion.