TUD Dresden University of Technology

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Annika Kolodziejczyk¹, Janine Hoffmann¹, Paula Cubillos¹, Mareike Albert¹

¹Center for Regenerative Therapies Dresden, TUD Dresden University of Technology

Here, we present the acute genetic manipulation of sliced human cortical organoids by electroporation. These cortical organoid models are particularly amenable to injection as ventricle-like structures can be readily identified after slicing, enabling the functional investigation of human cortical development, neurodevelopmental disorders, and cortical evolution.