University of Nebraska at Kearney

1 ARTICLES PUBLISHED IN JoVE

Behavior

Heat Tolerance Assays Using the Drosophila Activity Monitor System: A Guide to an Executable Application for Data Analysis

Blase Rokusek¹, Sunayn Cheku¹, Matthew Rokusek², Christopher J. Waples³, Lawrence Harshman⁴, Kimberly A. Carlson¹

¹Department of Biology, University of Nebraska at Kearney, ²School of Computing, University of Nebraska-Lincoln, ³Department of Psychology, University of Nebraska at Kearney, ⁴School of Biological Sciences, University of Nebraska-Lincoln

This report describes a method for measuring adult Drosophila melanogaster time to knockdown using a Drosophila Activity Monitor (DAM2) in response to an air conduction heat stressor within an incubator chamber. The DAM2 measures activity by recording individual fly movements as they cross an infrared beam. Data analysis is facilitated by a novel executable file created by the authors.