CSIC/Universidad Pablo de Olavide/JA

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches

Helena Sánchez-Gómez¹, Juan Garrido-Maraver¹, Patricia Rojas-Ríos²

¹Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide/JA, ²Departamento de Genética, Facultad de Biología, Universidad de Sevilla

Here, we provide a detailed protocol to perform live imaging of the asymmetric division of germline stem cells (GSCs) in the Drosophila ovarian niche. We use a transgenic line that ubiquitously expresses a green fluorescent protein (GFP) fusion of the spectrosome protein Par-1.