Plasmid Stability Analysis with Open-Source Droplet MicrofluidicsPierre Padilla-Huamantinco 1, Emerson Durán 1,2, Tobias Wenzel 1
1Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile, 2School of Veterinary Medicine, Faculty of Natural Resources and Veterinary Medicine, Santo Tomás University
An accessible, open-source microfluidic workflow is presented for the parallelized analysis of plasmid retention in bacteria. By employing fluorescence microscopy to quantify plasmid presence in single-cell microcolonies within gel microdroplets, this method provides a precise, accessible, and scalable alternative to traditional plate counting.
