Inducing the Entry of Third Stage Dispersal Juveniles of Bursaphelenchus xylophilus into Cryptobiosis through Osmotic RegulationLong Pan 1,2,3, Donglin Wang 1,2,3, Yutian Suo 1,2,3, Yaqi Wu 1,2,3, Wenyi Zhao 4, Jian Chen 5, Zhen Yu 5, Jiaping Zhao 1,2,3
11State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, 2Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, 3Tianjin Institute of Forestry Science, Chinese Academy of Forestry, 4Ningbo Yongsen Biotechnology Co. Ltd, 5Xiangshan Natural Resources and Planning Bureau of Zhejiang
The present protocol describes a method for inducing the dispersal of third-stage juveniles (pre-dauer juveniles, JⅢ) of Bursaphelenchus xylophilus into cryptobiosis through osmotic regulation of potassium chloride (KCl) ions. This method can provide technical support for research on the stress resistance mechanisms of B. xylophilus.
