Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical AnalysesAkiya Watakabe 1,2, Toshiki Tani 1, Hiroshi Abe 1, Henrik Skibbe 3, Noritaka Ichinohe 1,4, Tetsuo Yamamori 1,5
1Laboratory for Haptic Perception and Cognitive Physiology, RIKEN Center for Brain Science, 2Laboratory for Molecular Mechanism of Brain Development, RIKEN Center for Brain Science, 3Brain Image Analysis Unit, RIKEN Center for Brain Science, 4Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 5Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals
Serial two-photon tomography (STPT) imaging is a technique to image a mass of tissue in its three-dimensional shape by combining two-photon imaging with automatic stage control and microtome slicing. Here we describe a protocol for implementing it for marmoset brains to better understand their structural features.
