Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native GutPascal Benz 1, Masina Plenge 1, Simon Wagner 1, Gemma Mazzuoli-Weber 1,2
1Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, 2Center for Systems Neuroscience (ZSN)
This study outlines a protocol for generating 2D monolayers of porcine organoids derived from the small and large intestines. The growth of these monolayers is marked by increasing TEER values, indicating robust epithelial integrity. Additionally, these monolayers exhibit physiological secretory responses in Ussing chamber experiments following the application of forskolin.
