Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World SettingsLeonardo Gonçalves Gomes 1, Lucas de Figueiredo Soveral 1, Izadora Borgmann Frizzo 2, Thaise Brancher Soncini 1,3, Lívia Budziarek Eslabão 1, Daiana Silva Marcos Maniero 1, Isis Maia Apolinário de Mello 1, Jussara Kasuko Palmeiro 2, Thaís Cristine Marques Sincero 2, Oscar Bruna-Romero 4, Maria Marlene de Souza Pires 5, Carlos Rodrigo Zárate-Bladés 1
1Laboratory of Immunoregulation, iREG, Department of Microbiology, Immunology, and Parasitology, Center for Dysbiosis Control, Federal University of Santa Catarina, 2Laboratory of Applied Molecular Microbiology, MIMA, Department of Clinical Analysis, Center for Dysbiosis Control, Federal University of Santa Catarina, 3Carmela Dutra Maternity Hospital, 4Laboratory of Applied Immunology, LIA, Department of Microbiology, Immunology, and Parasitology, Center for Dysbiosis Control, Federal University of Santa Catarina, 5Laboratory of Metabolism and Nutrition, MenuLab, Department of Pediatrics, Center for Dysbiosis Control, Federal University of Santa Catarina
The article describes a protocol for reconstituting pasteurized donor milk microbiota using the mother's own milk microbiota in practical, real-world settings. It demonstrates effective bacterial growth and microbiome modulation, supporting the feasible application of this procedure within the routine care of a maternity hospital and its associated human milk bank.
