Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida

1 ARTICLES PUBLISHED IN JoVE

Genetics

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Felipe Muñoz-Guzmán^1,2,3, Pablo Quintrel^1,2, José Benavides-Parra^1,2, Catalina Muñoz-Tapia^2,3,4, Luis F. Larrondo^2,4, Francisco A. Cubillos^1,2,5

¹Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, ²ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), ³Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida, ⁴Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, ⁵ANID-Millennium Nucleus of Patagonian Limit of Life (LiLi)

This protocol employs a bioluminescent reporter, allowing measurements of transcriptional activity in Saccharomyces eubayanus to monitor the glucose-to-maltose transition, enabling real-time analysis of metabolic adaptations and supporting strain optimization for industrial fermentation under diverse conditions.