Optimizing Isolation and Purification of Murine Glomerular Mesangial CellsXinyu Zhai 1, Xiaoke Huang 2, Jingcao Huang 1, Yuhuan Zheng 1
1Department of Hematology/Institute of Hematology, West China Hospital, Sichuan University, 2Chengdu NO. 7 High School
This study developed an optimized protocol for the isolation of murine mesangial cells (MCs) and their ex vivo cell culture. These cells can be passaged multiple times, frozen, revived, and cultured without compromising cell growth or protein expression.
