Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor CellsAnna-Jasmina Donaubauer 1,2,3, Anna Schäfer 1, Simon Hundsdorfer 1, Rainer Fietkau 2,4, Udo S. Gaipl 1,2, Tina Jost 1,2
1Translational Radiobiology, Department of Radiation Oncology, Uniklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 2Comprehensive Cancer Center Erlangen-EMN, 3Bayerischerisches Zentrum für Krebsforschung, 4Department of Radiation Oncology, Uniklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg
The present protocol describes an experimental workflow that allows for the ex vivo analysis of human T cell stimulation in an allogenic co-culture system with pre-treated tumor cells.