Generation of Three-Dimensional Spheroids/Organoids from Two-Dimensional Cell Cultures Using a Novel Stamp DeviceHenrique Capistrano-Melo 1, Isaura Beatriz Borges Silva 1,2, Ivan Vieira Gama 3, Mari Cleide Sogayar 1,2, Marluce da Cunha Mantovani 1,4
1Cell and Molecular Therapy NUCEL Group, School of Medicine, University of São Paulo, 2Biochemistry Department, Chemistry Institute, University of São Paulo, 3Department of Polymers, Faculty of Technology of the State of São Paulo – Zona Leste (FATEC ZL), 4Technical Division for Teaching, Research and Innovation Support - DTAPEPI Biotechnology and Innovation Facility, School of Medicine, University of São Paulo
This study presents a cost-effective and efficient methodology for generating 3D cell structures using a stamp-based system to create microwells in agarose molds. The system promotes the formation of uniform spheroids/organoids, thus improving cell interactions. This approach reduces experimental variability and supports applications in drug testing and tissue engineering.
