An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell AnalysesLauryn Ruegg 1,2, Laura B. James-Allan 1,2, Gabriella DiBernardo 1,2, Christopher Ochoa 1,2,3, Yan-Ruide Li 4,5, Lili Yang 2,3,4,5,6,7, Sanaz Memarzadeh 1,2,3,7,8,9
1Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California, Los Angeles, 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 3Molecular Biology Institute, University of California, Los Angeles, 4Department of Bioengineering, University of California, Los Angeles, 5Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 6Parker Institute for Cancer Immunotherapy, University of California, Los Angeles, 7The Jonsson Comprehensive Cancer Center, University of California Los Angeles, 8The VA Greater Los Angeles Healthcare System, 9Department of Medical and Molecular Pharmacology, David Geffen School of Medicine, University of California, Los Angeles
This protocol describes a one-day method for the isolation of human fallopian tube epithelial cells. Isolated epithelial cells can be plated in 2-dimensional (2D) culture or dissociated into single-cell suspensions and utilized in downstream experiments, including flow cytometry and single-cell RNA sequencing.
