Isolation of Lung Retinoid-Containing Cells by Cell SortingSeth Calindas 1,2, Olga Ilnytska 1,3,4, Igor Shmarakov 1,3,4
1Department of Animal Sciences, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, 2Undergraduate Program in Biochemistry, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, 3Rutgers Center for Lipid Research, 4Rutgers Center for Environmental Exposures & Disease
The protocol described below is a simple and effective way to isolate retinoid-containing cells from highly heterogeneous lung cell populations by making use of specific retinoid autofluorescence and by employing fluorescent-activated cell sorting.