Establishment and Histological Analysis of Esophageal Organoids Modeling the Progression from Normal to Cancerous TissuesRucheng Liu *1,2,3, Lingxuan Zhu *1,2,3, Guoyu Cheng 1,2, Shaosen Zhang 1,2
1Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), 2Key Laboratory of Cancer Genomic Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), 3Changping Laboratory, Chinese Academy of Medical Sciences and Peking Union Medical College
The present protocol describes the establishment and histological analysis of esophageal organoid models that represent different stages of tumor progression. This method enables researchers to study changes in cellular morphology, spatial organization, and molecular marker expression patterns during the transition from normal to cancerous tissues.
