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University of Erlangen-Nuremberg

6 ARTICLES PUBLISHED IN JoVE

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Neuroscience

A Low Cost Setup for Behavioral Audiometry in Rodents
Konstantin Tziridis 1, Sönke Ahlf 1, Holger Schulze 1
1Experimental Otolaryngology, University of Erlangen-Nuremberg

A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.

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Bioengineering

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
Kristin Derlig 1, Andreas Gießl 2, Johann Helmut Brandstätter 2, Ralf Enz 1, Regina Dahlhaus 1
1Institute for Biochemistry, Emil-Fischer Centre, University of Erlangen-Nuremberg, 2Department of Biology, Animal Physiology, University of Erlangen-Nuremberg

Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.

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Education

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions
José R. Agudo 1, Jiwon Han 1, Jinyoung Park 1, Sinyoung Kwon 1, Soebiakto Loekman 2, Giovanni Luzi 1, Christoph Linderberger 3, Antonio Delgado 1,4, Andreas Wierschem 4
1Institute of Fluid Mechanics, FAU Busan Campus, University of Erlangen-Nuremberg, 2Institute of Chemical Reaction Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 3Institute of Bioprocess Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 4Institute of Fluid Mechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Two different methods for characterizing the incipient particle motion of a single bead as a function of the sediment bed geometry from laminar to turbulent flow are presented.

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Immunology and Infection

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
Emily Becker 1, Sebastian Schramm 1, Marie-Theres Binder 1, Clarissa Allner 1, Maximilian Wiendl 1, Clemens Neufert 1, Imke Atreya 1, Markus Neurath 1, Sebastian Zundler 1
1Department of Medicine 1, University of Erlangen-Nuremberg

Dynamic adhesion of immune cells to the vessel wall is a prerequisite for gut homing. Here, we present a protocol for a functional in vitro assay for the impact analysis of anti-integrin antibodies, chemokines or other factors on the dynamic cell adhesion of human cells using addressin-coated capillaries.

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Medicine

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
Anja Schulz-Kuhnt 1, Sebastian Zundler 1, Anika Grüneboom 2, Clemens Neufert 1, Stefan Wirtz 1, Markus F. Neurath 1, Imke Atreya 1
1Department of Medicine 1, University Hospital of Erlangen, 2Department of Medicine 3, University Hospital of Erlangen

The here introduced protocol allows characterization of the lung homing capacity of primary human lymphocytes under in vivo inflammatory conditions. Pulmonary infiltration of adoptively transferred human immune cells in a mouse model of allergic inflammation can be imaged and quantified by light-sheet fluorescence microscopy of chemically cleared lung tissue.

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Immunology and Infection

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
Luz DC. Martínez-Sánchez *1, Rashmita Pradhan *1, Phuong A. Ngo 1, Lena Erkert 1, Lukas S. Becker 1, Alastair J. Watson 2, Imke Atreya *1, Markus F. Neurath *1, Rocío López-Posadas *1
1Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, 2Norwich Medical School, University of East Anglia, Norwich Research Park

Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.

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