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Korea Advanced Institute of Science and Technology

9 ARTICLES PUBLISHED IN JoVE

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Biology

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
Adeola F. Adewola 1, Yong Wang 1, Tricia Harvat 1, David T. Eddington 2, Dongyoung Lee 1, Jose Oberholzer 1,2
1Department of Surgery, University of Illinois, Chicago, 2Department of Bioengineering, University of Illinois, Chicago

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes.

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Engineering

Experimental Implementation of a New Composite Fabrication Method: Exposing Bare Fibers on the Composite Surface by the Soft Layer Method
Dongyoung Lee 1, Dai Gil Lee 1, Jun Woo Lim 2
1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 2LANL-CBNU Engineering Institute Korea, Chonbuk National University

A protocol to expose bare fibers on the composite surface by eliminating resin rich area is presented. The fibers are exposed during fabrication of the composites, not by the post surface treatment. The exposed carbon composites exhibit high electrical conductivity in the through-thickness direction and high mechanical property.

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Neuroscience

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus
Anvar Sariev 1,2, Jinho Chung 1, Dajung Jung 1,3, Farnaz Sharif 1,2, Ju-Young Lee 1,2, Soyoun Kim 1, Sebastien Royer 1,2
1Center for Functional Connectomics, Korea Institute of Science and Technology, 2Biomedical department, KIST school, University of Science and Technology, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology

We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.

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Neuroscience

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes
Youkyeong Gloria Byun 1, Won-Suk Chung 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.

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Genetics

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
Hogyu David Seo 1, Daeyoup Lee 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

This article describes a detailed methodology for random mutagenesis of a target gene in fission yeast. As an example, we target rpt4+, which encodes a subunit of the 19S proteasome, and screen for mutations that destabilize heterochromatin.

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Immunology and Infection

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
Jonghee Yoon 1, YoungJu Jo 2,3,4,7, Young Seo Kim 3,4,5, Yeongjin Yu 2,3, Jiyeon Park 6, Sumin Lee 4, Wei Sun Park 2,3, YongKeun Park 2,3,4
1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 7Department of Applied Physics, Stanford University

We describe a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and a machine learning algorithm. Measurements of 3D refractive index tomograms of lymphocytes present 3D morphological and biochemical information for individual cells, which is then analyzed with a machine-learning algorithm for identification of cell types.

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Engineering

Preparation of Graphene Liquid Cells for the Observation of Lithium-ion Battery Material
Joon Ha Chang *1, Jun Young Cheong *1, Hyeon Kook Seo 1, Il-Doo Kim 1, Jong Min Yuk 1
1Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology

Here, we present a protocol for the fabrication and preparation of a graphene liquid cell for in situ transmission electron microscopy observation, along with a synthesis of electrode materials and electrochemical battery cell tests.

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Bioengineering

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
Hwanseok Jang 1, Jongseong Kim 1, Jennifer H. Shin 2, Jeffrey J. Fredberg 3, Chan Young Park 3, Yongdoo Park 1
1Department of Biomedical Sciences, Korea University, 2Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 3Department of Environmental Health, Harvard T.H. Chan School of Public Health

Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical gradient.

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Medicine

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window
Inwon Park 1, Pilhan Kim 2,3
1Department of Emergency Medicine, Seoul National University Bundang Hospital, 2Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, 3KI for Health Science and Technology, Korea Advanced Institute of Science and Technology

In vivo high-resolution imaging of the pancreas was facilitated with the pancreatic intravital imaging window.

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