University of the Balearic Islands

3 ARTICLES PUBLISHED IN JoVE

Chemistry

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Alexandros Lamprou¹, Hongxia Wang^1,4, Adeela Saeed², Frantisek Svec¹, David Britt¹, Fernando Maya³

¹The Molecular Foundry, E. O. Lawrence Berkeley National Laboratory, ²Institute of Chemical Sciences, Bahauddin Zakariya University, ³Department of Chemistry, University of the Balearic Islands, ⁴Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology

A procedure for the preparation of porous hybrid separation media composed of a macroporous polymer monolith internally coated by a high surface area microporous coordination polymer is presented.

Bioengineering

Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants

Maria Antònia Forteza-Genestra^1,2, Miquel Antich-Rosselló^1,2, Francisco Gabriel Ortega^1,2, Guillem Ramis-Munar³, Javier Calvo^1,2,4, Antoni Gayà^1,2,4, Marta Monjo^1,2, Joana Maria Ramis^1,2

¹Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, ²Health Research Institute of the Balearic Islands (IdISBa), ³Cellomics Unit, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, ⁴Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB)

Here, we present a protocol to label platelet lysate-derived extracellular vesicles to monitor their migration and uptake in cartilage explants used as a model for osteoarthritis.

Bioengineering

Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

Miquel Antich-Rosselló^1,2, Maria Antònia Forteza-Genestra^1,2, Javier Calvo^1,2,3, Antoni Gayà^1,2,3, Marta Monjo^1,2,4, Joana Maria Ramis^1,2,4

¹Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, ²Health Research Institute of the Balearic Islands (IdISBa), ³Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB), ⁴Department of Fundamental Biology and Health Sciences, University of the Balearic Islands

Here, we present a method for the isolation of Extracellular Vesicles (EVs) derived from the platelet lysates (PL) and their use for coating titanium (Ti) implant surfaces. We describe the drop casting coating method, the EVs release profile from the surfaces, and in vitro biocompatibility of EVs coated Ti surfaces.