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Queen's University Belfast

4 ARTICLES PUBLISHED IN JoVE

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Biology

Preparation of Highly Coupled Rat Heart Mitochondria
Irina Gostimskaya 1, Alexander Galkin 2
1Faculty of Life Sciences, University of Manchester, 2School of Biological Sciences, Queen's University Belfast

We describe а protocol for isolation of pure, highly coupled rat heart mitochondria for functional or structural studies of cellular bioenergetics, biophysical measurements, proteomics or mitochondrial DNA and lipids analysis.

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Biochemistry

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Myriam Jaraíz-Rodríguez 1, Ana González-Sánchez 1,2, Laura García-Vicente 1, Jose M. Medina 1, Arantxa Tabernero 1
1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast

This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.

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Bioengineering

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
Calvin C. L. Cheung 1, Guanglong Ma 1, Amalia Ruiz 1, Wafa T. Al-Jamal 1
1School of Pharmacy, Queen's University Belfast

The protocol presents the optimized parameters for preparing thermosensitive liposomes using the staggered herringbone micromixer microfluidics device. This also allows co-encapsulation of doxorubicin and indocyanine green into the liposomes and the photothermal-triggered release of doxorubicin for controlled/triggered drug release.

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Medicine

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
Michael A. Pearen *1, Hong Kiat Lim *1, Francis D. Gratte 2,3, Manuel A. Fernandez-Rojo 1,4,5, Sujeevi K. Nawaratna 6, Geoffrey N. Gobert 7, John K. Olynyk 8,9, Janina E. E. Tirnitz-Parker 2,10, Grant A. Ramm 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia

This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.

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