Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

15.3K Views

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12:36 min

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March 14th, 2020

DOI :

10.3791/60992-v

March 14th, 2020

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Michael A. Pearen*¹, Hong Kiat Lim*¹, Francis D. Gratte²^,³, Manuel A. Fernandez-Rojo¹^,⁴^,⁵, Sujeevi K. Nawaratna⁶, Geoffrey N. Gobert⁷, John K. Olynyk⁸^,⁹, Janina E. E. Tirnitz-Parker²^,¹⁰, Grant A. Ramm¹^,⁴

¹Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, ²School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, ³School of Veterinary and Life Sciences, Murdoch University, ⁴School of Medicine, The University of Queensland, ⁵Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, ⁶School of Medicine, Griffith University, ⁷School of Biological Sciences, Queen's University Belfast, ⁸Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, ⁹School of Medical and Health Sciences, Edith Cowan University, ¹⁰Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia

Summary

Explore More Videos

Precision cut Liver Slices

Ex Vivo Model

Liver Biology

In Vivo Studies

In Vitro Cell Culture

Tissue Culture

Cholestatic Liver Injury

Chapters in this video

0:00

Introduction

1:23

Setup of the Vibratome

3:15

Liver Removal and Preparation

6:07

Production of Liver Slices

10:14

Results: Viability, Collagen, and Effects of Selected Bile Acids

11:45

Conclusion

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