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Wistar Institute

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
Melanie R. Rutkowski *1, Michael J. Allegrezza *1, Nikolaos Svoronos 1, Amelia J. Tesone 1, Tom L. Stephen 1, Alfredo Perales-Puchalt 1, Jenny Nguyen 1, Paul J. Zhang 2, Steven N. Fiering 3, Julia Tchou 4,5,6, Jose R. Conejo-Garcia 1
1Tumor Microenvironment and Metastasis Program, Wistar Institute, 2Department of Pathology and Lab Medicine, Perelman School of Medicine, University of Pennsylvania, 3Department of Microbiology and Immunology and Department of Genetics, Geisel School of Medicine at Dartmouth, 4Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine, University of Pennsylvania, 5Rena Rowan Breast Center, Abramson Cancer Center, University of Pennsylvania, 6Center for Advanced Medicine, University of Pennsylvania

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

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Cancer Research

A Melanoma Patient-Derived Xenograft Model
Min Xiao 1, Vito W. Rebecca 1, Meenhard Herlyn 1
1Wistar Institute

Patient-derived xenograft (PDX) models more robustly recapitulate melanoma molecular and biological features and are more predictive of therapy response compared to traditional plastic tissue culture-based assays. Here we describe our standard operating protocol for the establishment of new PDX models and the characterization/experimentation of existing PDX models.

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Immunology and Infection

Gene Editing of Primary Rhesus Macaque B Cells
Harald Hartweger 1, Rajeev Gautam 2, Yoshiaki Nishimura 2, Fabian Schmidt 3,5, Kai-Hui Yao 1, Amelia Escolano 1,6, Mila Jankovic 1, Malcolm A. Martin 2, Michel C. Nussenzweig 1,4
1Laboratory of Molecular Immunology, The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Retrovirology, The Rockefeller University, 4Howard Hughes Medical Institute, The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center, Wistar Institute

We present a method for culturing and gene editing primary rhesus macaque B cells using CRISPR/Cas9 and recombinant adeno-associated virus serotype 6 for the study of B cell therapies.

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