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Faculdade de Medicina da Universidade de Lisboa

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
Cláudia A. Valente 1,2, Francisco J. Meda *1,2, Mafalda Carvalho *1,2, Ana M. Sebastião 1,2
1Instituto de Farmacologia e Neurociências, Faculdade de Medicina da Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa

Here, we describe the preparation of rhinal cortex-hippocampus organotypic slices. Under a gradual and controlled deprivation of serum, these slices depict evolving epileptic-like events and can be considered an ex vivo model of epileptogenesis. This system represents an excellent tool for monitoring the dynamics of spontaneous activity, as well as for assessing the progression of neuroinflammatory features throughout the course of epileptogenesis.

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Biology

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
Madalena R. de Almeida 1, Eduardo Gameiro 1, Sérgio F. de Almeida 1, Robert M. Martin 1
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa

This protocol presents a new reporter gene system and the experimental setup to detect transcription at DNA double-strand breaks with single-molecule sensitivity.

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Medicine

Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model
Pedro S. de Oliveira 1,2, Filipe Rocha 1, Inês Sofia Vala 1, Paula de Oliveira 1, Augusto Ministro 1,3, Susana Constantino Rosa Santos 1
1Cardiovascular Center of the University of Lisbon (CCUL@RISE), Faculdade de Medicina da Universidade de Lisboa, 2Department of Urology, Centro Hospitalar Universitário Lisboa Norte, 3Department of Vascular Surgery, Centro Hospitalar Universitário Lisboa Norte

In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.

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Neuroscience

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
Ana Filipa Isidro 1, Alexandra M. Medeiros 2, Isaura Martins 1, Dalila Neves-Silva 1, Leonor Saúde 1,3, César S. Mendes 2
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 2iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, NMS|FCM, Universidade Nova de Lisboa, 3Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa

An experimental pipeline to quantitatively describe the locomotor pattern of freely walking mice using the MouseWalker (MW) toolbox is provided, ranging from initial video recordings and tracking to post-quantification analysis. A spinal cord contusion injury model in mice is employed to demonstrate the usefulness of the MW system.

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