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Universitat Politècnica de València

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Sandwich-like Microenvironments to Harness Cell/Material Interactions
José Ballester-Beltrán 1,2, Myriam Lebourg 1,3, Manuel Salmerón-Sánchez 2
1Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 2Division of Biomedical Engineering, School of Engineering, University of Glasgow, 3Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)

The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.

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Developmental Biology

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
Juan G. Herranz-Jusdado 1, Eszter Kása 2, Tímea Kollár 2, Víctor Gallego 1, David S. Peñaranda 1, Christoffer Rozenfeld 1, Luz Pérez 1, Ákos Horváth 2, Juan F. Asturiano 1
1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Szent Istvan University

Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

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Engineering

Design and Fabrication of an Optical Fiber Made of Water
Mark L. Douvidzon 1, Shai Maayani 2, Leopoldo L. Martin 3, Tal Carmon 4
1Department of Nanoscience and Nanotechnology, Russell Berrie Nanotechnology Institute (RBNI), Technion - Israel Institute of Technology, 2Department of Material Sciences and Engineering, MIT, 3Centro de Tecnologia Nanofotónica, Universitat Politècnica de València, 4Department of Mechanical Engineering, Technion - Israel Institute of Technology

This protocol describes the design and manufacture of a water bridge and its activation as a water fiber. The experiment demonstrates that capillary resonances of the water fiber modulate its optical transmission.

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Environment

Forced Flowering in Mandarin Trees under Phytotron Conditions
Alfonso Garmendia 1, Roberto Beltrán 2, Carlos Zornoza 3, Francisco J. García-Breijo 2, José Reig 4, María Dolores Raigón 5, Hugo Merle 2
1Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, 2Departamento de Ecosistemas Agroforestales, Universitat Politècnica de València, 3S.A. Explotaciones Agrícolas Serrano (SAEAS), 4Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Jardín Botánico Universitat de València, 5Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València

Here, we present a protocol to force flowering in mandarin trees under phytotron conditions. Water stress, high illuminance and a simulated spring photoperiod allowed viable flowers to be obtained in a short period of time. This methodology allows researchers to have several flowering periods in 1 year.

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Bioengineering

Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts
Néstor J. Martínez-Hernández 1, Lara Milián-Medina 2, Jorge Mas-Estellés 3, José L. Monroy-Antón 4, José L. López-Villalobos 5, David Hervás-Marín 6, Amparo Roig-Bataller 1, Manuel Mata-Roig 2,7,8
1Thoracic Surgery Department, Hospital Universitari de la Ribera, 2Pathology Department, Universitat de Valencia Facultat de Medicina i Odontologia, 3Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 4Radiation Oncology, Hospital Universitari de la Ribera, 5Thoracic Surgery Department, Hospitales Universitarios Virgen del Rocío, 6Department of Applied Statistics and Operations Research and Quality, Universitat Politècnica de València, 7Networking Research Center on Respiratory Diseases (CIBERER), 8INCLIVA Biomedical Research Institute

Obtaining sterilization is essential for tracheal tissue transplant. Herein, we present a sterilization protocol using low-dose gamma irradiation that is fully tolerated by organs.

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