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Translational Genomics Research Institute

1 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
Pawan Noel 1, Ruben Muñoz 1, George W. Rogers 2, Andrew Neilson 2, Daniel D. Von Hoff 1, Haiyong Han 1
1Molecular Medicine Division, Translational Genomics Research Institute, 2Agilent Technologies

Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.

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